Electrophoresis Matrix
•Solid matrix is generally agarose (larger pore size) or polyacrylamide (smaller pore size)
•Buffer provides ions for current while maintaining constant pH
•For capillary electrophoresis, buffer serves as matrix
•Buffer provides ions for current while maintaining constant pH
•For capillary electrophoresis, buffer serves as matrix
Preparation
Gel electrophoresis set
1.Gel tray
2.Comb
3.Gel chamber
4.Buffer
5.Electrical supply
1.Gel tray
2.Comb
3.Gel chamber
4.Buffer
5.Electrical supply